Hamad Rafique

4 Questions 5 Answers 0 Followers

Questions related from Hamad Rafique

How can dissolve hydrophobic peptides to analyse their bioactivities in cell lines and mice study?

I am working on structure-activity relationship of oat protein derived peptides . The peptides sequence have poor water solubility, so i want to know which solvent is good to dissolve it, without...

01 November 2022 1,822 7 View

Problem facing about Antioxidant enzyme assays in PC12 cells ?

I have used difference concentration of hydrogen peroxide to develop an oxidative stress model in Pc12 cells. The cell viability was gradually decreased as concentration of H2O2 increased ( result...

23 June 2022 8,474 0 View

How can remove non protein impurities from enzymatic hydrolyzed protein (to get purified hydrolysates) by using column chromatography????

I am looking for a method to purify protein hydrolysates from impurities. I have used enzymes to hydrolyzed ultrasound pretreated oat protein. Initially, the protein content of isolates was about...

07 April 2022 3,901 0 View

How can we calculate enzyme to substrate ratio according to units??

I want to hydrolyze oat protein by using enzyme Alcalase 2.4 L ( 2.4 AU g). how can we calculate enzyme / substrate ratio 0.3 AU g of protein??

10 August 2021 5,072 3 View