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Questions related from Hafiz Ishfaq Ahmad
I need to predict pseudo-genes in the vertebrate species. I want to know how can identify or suppose that the specific gene in the genome is a pseudo or not? I need detailed and simple method to...
08 August 2019 6,685 2 View
Dear researchers, I want to make 5ml, 2mM L-Tyrosine, and L-Dopa substrate solutions separately. I purchased these chemical from Sigma. The L-Tyrosine and L-Dopa MW weights are 181.19g/mol and...
03 March 2018 3,640 0 View
I am trying to express sheep Tyrosinase protein with wild type and mutant type in E. coli bacteria. I have cloned my genes in pGEX-6P-1 vector. I have confirmed the cloning of gene by sequencing....
11 November 2017 378 3 View
Dear friends, I am cloning a gene in DH5a cells using pGEX-6P-1 vector. I did double digestion of both gene and vector with EcoR1 and Xho1 with fastdigest enzymes. I got the colonies after...
08 August 2017 9,525 8 View
Hello Friends! I am doing protein expression in E. coli (BL21 (DE3 CC). I want to extract and purify protein from bacterial culture. I need your suggestions and your experience for protein...
05 May 2017 5,889 6 View
I have problem with plasmid digestion. I can see one band of uncut plasmid and after double digestion with EcoR1 and Xho1 can see multiple bands. What is problem with plasmid? Is my plasmid...
11 November 2016 6,660 4 View
Hello Friends, I am trying to transform 1.6kb gene and 3kb plasmid into Dh5a competent cells. I digested gene and plasmid both with double digestion, EcoR1 and Xho1. I confirmed digestion from gel...
11 November 2016 7,726 3 View
