How to make 2mM substrate solution?

Dear researchers, I want to make 5ml, 2mM L-Tyrosine, and L-Dopa substrate solutions separately. I purchased these chemical from Sigma. The L-Tyrosine and L-Dopa MW weights are 181.19g/mol and...

02 March 2018 3,584 0 View

Facing Problem in Sheep Tyrosinase protein expression in E.coli?

I am trying to express sheep Tyrosinase protein with wild type and mutant type in E. coli bacteria. I have cloned my genes in pGEX-6P-1 vector. I have confirmed the cloning of gene by sequencing....

10 November 2017 327 3 View

Gene Cloning and Expression

Dear friends, I am cloning a gene in DH5a cells using pGEX-6P-1 vector. I did double digestion of both gene and vector with EcoR1 and Xho1 with fastdigest enzymes. I got the colonies after...

07 August 2017 9,471 8 View

Protein extraction and purification

Hello Friends! I am doing protein expression in E. coli (BL21 (DE3 CC). I want to extract and purify protein from bacterial culture. I need your suggestions and your experience for protein...

04 May 2017 5,830 6 View

Hello friends,

I have problem with plasmid digestion. I can see one band of uncut plasmid and after double digestion with EcoR1 and Xho1 can see multiple bands. What is problem with plasmid? Is my plasmid...

10 November 2016 6,611 4 View

Ligation or transformation problem

Hello Friends, I am trying to transform 1.6kb gene and 3kb plasmid into Dh5a competent cells. I digested gene and plasmid both with double digestion, EcoR1 and Xho1. I confirmed digestion from gel...

10 November 2016 7,665 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Can anyone provide the Support vector regression code structure of LIBSVM MATLAB or python ?

Please, if Gaussian Parameter optimization is possible in this code structure

28 February 2021 278 3 View

How to modify Johnson cook equation in ABAQUS?

I wanted to add an extra parameter to the existing Johnson cook equation in the ABAQUS simulation. How to add those extra parameters. Ref: A modified Johnson-Cook material model with strain...

28 February 2021 4,841 3 View

No module named 'tensorflow.keras.backend.tensorflow_backend' ?

System Description Cuda 11.2 GeForce GTX 1080ti tensorflow version 2.4.1 keras version 2.2.0 python version 3.8 I was getting the below error when I try to run my code on the...

25 February 2021 6,673 2 View

Implementation of chance-constrained optimization problem via scenario approach?

23 February 2021 7,826 2 View

Bayesian decision trees and bayesian regression trees in Python and/or R?

I am looking for implementation of bayesian decision trees and bayesian regression trees in Python and/or R.. Do you know if the existing libraries support this

23 February 2021 6,689 3 View

Are there packages (R or Python) for simple models (bucket?) to assess soil water content?

Hi everyone. I am researching droguht effects on soil water drought. I am looking for already implemented functions in R or Python to estimate soil water content from precipitation,...

21 February 2021 2,084 1 View

How can I retrieve FASTA sequence from gene ID or UID of a Gene ? I have GeneBank IDs ?

I treid to use Entrez Batch, but it redirects me to NCBI site. Is there any script of Linux or Python through which I can download Fasta sequence of list of gene ID ?

21 February 2021 7,783 12 View