Gunes-Helene Isitan

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Questions related from Gunes-Helene Isitan

How to get to Archaea DNA?

Hi! anyone knows of the best methods to get to Archaea DNA? Their cells are tough to break open! I you think I should go the beadbeating way, mind suggesting which glass size to use, and a time?...

12 December 2018 881 0 View

What is your best trick to get those pesky and stubborn unknown DNA samples to amplify?

Hi! I have been growing colonies from mixed gut samples, and while most amplified easily, I am still left with quite a few that managed to avoid amplifying. I tried regular Taq with bacterial 16s...

12 December 2018 10,014 0 View

Spin columns: difference between those for plasmids, pcr product, virus and genomic DNA??

Hi! I've been looking for replacement spin columns for purifying PCR product from 16s bacterial DNA. I have found several "different" ones online: some for plasmid DNA, some for genomic DNA, some...

07 July 2018 5,237 5 View

Which ladder should I use with circular MtDNA?

Hi! Does anyone knows what type of ladder I sould use for circular mtDNA in an agarose gel? I am trying to isolate mammalian mitochondrial DNA from nuclear DNA using a 0.5% agarose gel,...

06 June 2018 9,499 6 View

Mammalian mtDNA isolation: sucrose gradient or spin columns?

Hi! I've been trying to isolate complete mtDNA plasmids from cheek cells (16.5kbp). Once the nuclear DNA is pelleted and the supernatant containing the mtDNA is transfered in a new tube, pelleted,...

05 May 2018 7,768 4 View

DNA extraction protocol for anaerobic bacteria? (Gram + and -)?

Hi! I have been trying to extract DNA from anaerobic bacteria (both gram positive and negative) using the lyzozyme/proteinase K/sds/chloroform/isopropanol protocol, but have repeatly failed to get...

04 April 2018 8,509 5 View

Pharmacia EPS 3500 XL electrophoresis power supply leads?

Hi! Ok, that may be a very basic question to ask this community, but I have searched and searched and searched some more, and can't seem to find the answer! What are the required adapters to...

04 April 2018 7,073 0 View

Possible pathogens present in healthy people's gut microbiota?

Hi! I have been asked (Health & Safety department) to provide a list of possible pathogens that could be grown from healthy human stools as a risk assessment for an upcoming research-creation...

01 January 2018 6,268 2 View

Paramecium and electricity: why do they stop responding to galvanotaxis after a while?

Hi, I am working on an interactive art installation that offers contact zones between human and non-human. In this specific case, my non-human are Paramecium Caudatum and Multimicronucleatum. I am...

03 March 2016 6,350 6 View