23 Questions 28 Answers 0 Followers
Questions related from Guillermo Domínguez Huerta
Hi, I'd like to know what difference makes using FULL PROTEIN SEQUENCES versus PROTEIN CONSERVED DOMAIN SEQUENCES in order to make a phylogenetic tree. In my case, the goal is to put in...
04 April 2019 8,829 5 View
Hi all, I am trying to perform self-training with GeneMarkS to improve protein calling from virus genomes and transcripts. Could someone tell me if it is correct what I am doing? First, I...
10 October 2018 9,974 0 View
Does anyone know any good qPCR primers for MS2 - using SYBR Green qPCR kit!!
09 September 2018 3,845 2 View
Hi all, I'm looking for the expert opinion from a chemist, since I am only a biologist. I have a set of samples made in the following manner: many litters of seawater were...
08 August 2018 2,891 3 View
Hi all, would you say that rnaSPADES is a better assembler to discover RNA viruses from read datasets THAN SPADES? thanks!
07 July 2018 8,648 2 View
Hi all, I looking for RNA viruses from datasets. I already performed a blastx of the contigs generated against the database of all reference RNA virus proteins download from GenBank. Now I'd...
06 June 2018 10,086 3 View
Hi all, I just did my first RP-SISPA. I extracted RNA from two virus species by different methods. One virus genome is ssRNA of 3 kb and the other virus genome is segments of dsRNA (3, 4 and 6...
04 April 2018 5,449 4 View
Hi all, I've been having trouble increasing the efficiency of a couple of primers using a standard curve. - The standard curve is built with log10 dilutions of plasmid into which the amplicon...
03 March 2018 9,653 7 View
Hi all, I wondering about bioinformatic tools to capture "RNA viruses" from environmental sequence datasets. dsDNA phages are pretty abundant and have relatively conserved hallmark genes....
03 March 2018 6,996 6 View
Hi all, I looking for literature evidence for this phenomenon. Apparently, RNA (I'm not sure if also DNA) is cleavaged in a non-enzymatic manner by iron. I would need some theory to support...
01 January 2018 7,419 3 View
Hi all, I know this is a typical phenomenon, but after reading I'm not getting a convincing explanation. I prepared 4 L of a buffer containing 10 mM KH2PO4 and 1 mM MgSO4*7H2O. This time I...
01 January 2018 2,694 4 View
Get rid of the iron from my samples. Iron chelating capacity of EDTA? Briefly, I have samples consisting of resuspended viruses precipitated with FeCl3. After flocculation of viruses, I resuspend...
12 December 2017 3,303 8 View
Beyond human pathogenic viruses (enteroviruses, hepatitis A virus, rotavirus, norovirus)..., which other riboviruses are expected to found in freshwaters from an urban river? I'm looking for...
12 December 2017 1,088 2 View
I know that SYBR cannot distinguish between dsDNA and dsRNA, but how efficient is SYBR binding RNA molecules? Do you know other method for counting RNA viruses? maybe other dyes?
10 October 2017 8,465 2 View
Hi, Imagine you got a mutant library of a plasmid DNA generated by random mutagenesis and affecting the whole plasmid sequence. This mutant library is going to be used to transform bacteria and...
05 May 2017 5,327 6 View
The evolution of a population of small rodents has been studied. The size of the population to each time n is noted as Pn. The integer n designs the time expressed in months, and Pn is expressed...
12 December 2016 7,415 0 View
I am searching for opportunities for doing my first postdoc stage. And I would love to get that expertise needed! Any advice would be appreciated! many thanks!
09 September 2016 7,398 4 View
I mean, programming, bioinformatics, quatitative biology, etc.
06 June 2016 8,718 1 View
Proper Evolutionary Biology, not slightly related! thx!
06 June 2016 1,113 4 View
I have finished my PhD in Biology, with a thesis about molecular evolution of a virus (molecular biology and routine bioninformatics methodology). Only one publication (soon, but still waiting) by...
06 June 2016 9,455 2 View
Hi everybody! I do very usually agrobacterium culture for agroinoculation of plants. I mean I can get virus-infected plants through introduction of agrobcateria with a needle. Normally, I start a...
12 December 2011 7,845 0 View
12 December 2011 4,278 0 View
Hi all, I'm looking for viruses in metatranscriptomes from environmental samples. After assembly of the reads (Illumina HiSeq RNA), protein sequences were predicted using FragGenescan. I run CAT...
01 January 1970 6,988 3 View
