Gisela Leticia Isasi Rivas

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Since SMAC/DIABLO has different variants and isoforms, which is the most commonly used?

I’m looking for a SMAC/DIABLO sequence to insert into a viral vector. I realized there are different variants, isoforms, and even peptide fragments that might be viable. Are some of these more...

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What is the best approach to analyze a treatment in a tumor spheroid in terms of fluorescence?

I am looking for the best way to analyze a tumor spheroid in terms of fluorescence and viability. I believe it would be useful to evaluate it by quantifying the area occupied by the fluorochrome,...

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What is the optimal size of a tumor spheroid for conducting drug screening assays with different compounds?

I noticed that spheroids vary depending on the cell type. When I seed the same number of cells for different types, the resulting spheroids are of different sizes. I need to understand whether the...

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Does cholera toxin inhibit fibroblast cells growth?

I'm working with primary cultures, but fibroblast cells hinder efficient recovery. I have read that cholera toxin can be used to inhibit fibroblast growth.

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