Giovanni De Nola

5 Questions 7 Answers 0 Followers

Questions related from Giovanni De Nola

Is it possible to produce liposomes or liposomes-like structures starting from living cells?

Hello! I am pretty new to this kind of topic (lisosomes and artificial membranes in general) and I was wandering if anyone here knows or can suggest me some protocol in order to get this kind of...

14 June 2016 2,603 6 View

Can anyone recommend a good protocol for cross-linking proteins?

I am working with several protein-proten and protein-heparin complexes. After the complex formation in the tube I can see a shift in gel filtration column but the complexes are not stable enough...

09 July 2015 9,860 6 View

How can I determine L-arginine concentration in a sample?

I am using L-arginine in my refolding buffer. After the time required for the refolding to happen I have to dialyse my sample in order to eliminate the arginine that would prevent the binding of...

20 January 2015 814 1 View

Does anyone have experience in setting up crystallization screening using Taguchi Method?Is it a valid method to be applied to this kind of screening?

Taguchi Method and crystallization

10 September 2014 1,089 2 View

Experimental technique to determine if protein has free cysteine?

I need to determine if my purified protein has or not a free cysteine, do anyone of you have a good protocol to suggest me? I know that there are a lot of kits made for this purpose but since this...

06 February 2014 6,916 11 View