Ghulam Abbas

7 Questions 3 Answers 0 Followers

Questions related from Ghulam Abbas

How do we interpret Nernst equation to determine the glutathione potential in mitochondria or cytosol?

I've followed many articles to find out the relationship of various concentrations and the potential of GSH or GSSG in different cellular organelles but it's quite confusing for me to find out how...

03 June 2021 6,524 5 View

Is mitochondria evenly distributed in leukemia cells? Do they have regular shape as for normal cells?

Currently, I am facing some issues related mitochondrial localized expression of my fluorescent protein. So, if someone have any idea about the above query, it would be wonderful.

15 October 2020 6,571 1 View

How transfected DNA is transported into the nucleus, to get stable integration?

What kind of mechanisms are involved in the integration and transportation of transfected DNA/Gene to the nucleus?

09 October 2020 2,401 2 View

What is the best concentration of Polybrene during lentivirus infection in AML cells?

I have tried different concentrations of polybrene during experiment but as AML cells are difficult to transfect so could not determine which is the best concentration? To what extent it causes...

06 November 2019 4,716 3 View

What is the optimum time to harvest Lentivirus from HEK293T cells after transfection?

I have tried till now 72 hours harvesting but I'm not sure which time would be the optimum for maximum potency? Can somebody please guide me in this regard?

06 November 2019 2,176 3 View

What are the suggested methods to improve the plasmid transfection efficiency in AML suspension cell lines?

I gave tried lentiviral infection system but the transfection efficiency is not very good? Kindly suggest me some other methods as well as how can I improve the transfection efficiency in...

24 June 2019 3,586 4 View

What are the effective ways to synchronize cell cycle? Does the serum deprivation will work for cancerous cell lines?

How it will work for adherent cells and cells in suspension? Is there any cells toxicity probability?

11 January 2019 1,179 3 View