Gergely N. Nagy

4 Questions 6 Answers 0 Followers

Questions related from Gergely N. Nagy

Overexpression of a Fe-S cluster protein in E coli?

I would like to achieve heterologous overexpression of a mycobacterial enzyme in E coli that harbors a Fe-S cluster. Unfortunately, the expressed and purified His-tagged protein apparently lacked...

05 May 2016 223 3 View

Which crosslinker do you advise for the detection of a homodimer in solution?

I wish to perform a chemical crosslink experiment on a homodimer protein with low thermal stability. Due to thermal stability issues, the desired crosslink needs to be done

09 September 2014 8,247 3 View

How can I get rid of air in an AKTA purifier inlet tube?

Accidentally air was sucked in by the pumpwash protocol as the pump inlet was not immersed in solvent. Currently the pump is not capable of sucking up solvent whaterver hard it tries. Could you...

08 August 2014 3,680 8 View

How to connect Superdex 200 column for reverse-flow?

I tried to clean my Superdex 200 AKTA gel filtration column after an event of protein sample aggregation on the column during gel filtration. Unfortunately, the column seems to be literally...

05 May 2014 3,414 7 View