Frances Lee

5 Questions 4 Answers 0 Followers

Questions related from Frances Lee

BD CBA cytokine kit assay use with BDFACSDiva and Flowjo?

Does anyone know if the CBA cytokine assay offered by BDbiosciences is compatible to run with BDFACSDiva software on a flow cytometer (vs the BDFACSSuite software that is recommended in the user...

16 January 2019 5,804 5 View

Positive Vs Negative T Cell Isolation Kits (STEMCELL EasySep)?

Hello! I'd appreciate any input on the advantages/disadvantages of using a positive or negative T cell isolation kits (STEMCELL EasySep) for in vitro assays. I would like to isolate T cells...

12 December 2018 5,365 4 View

When performing multicolor flow cytometry can one use several different color panels in one experiment?

So let's say I have 3 samples and I am interested in testing different fluorophore-conjugated antibodies against certain antigens by using 12 different colors. Howeveer, aach sample only has 10...

06 April 2018 4,206 2 View

Which species normal serum to use for IHC?

I am working on some immunohistochemistry staining of paraffin embedded tissues and am curious to know if there are any guidelines in choosing the species of the normal serum used for a blocking...

18 January 2018 1,858 3 View

When staining targets from 2 different species for flow cytometry, can you use 2 different primary Abs against different species in the same sample?

(if you staining for a mouse target and human target within one mouse (ex, chimera), can this be stained in the same tissue sample? Or should samples be stained separately?)

10 August 2017 7,271 2 View