Fletcher Del Valle

5 Questions 9 Answers 0 Followers

Questions related from Fletcher Del Valle

In Clustal Emboss Transeq, which frames (results) should I choose to align?

I used clustal emboss transeq to convert 3 viral DNAs into amino acid. I checked 6 frames in the choices, and I got got 6 results. Should I align all 6 frames of the 3 viruses? Or should I select...

20 February 2020 288 2 View

Good day, what could be the reason why PCR products in the gel do not migrate?

PCR gel electrophoresis PCR trouble-shooting

17 April 2016 1,056 6 View

I am using vaccine virus as positive control in PCR, my primer has a 300-bp product. Would a primer with a bigger bp product (700-800bp) be better?

Chicken anemia virus PCR vaccine virus positive control in PCR with very weak bands is a primer with a bigger bp-product better?

28 September 2015 8,584 2 View

I am trying to use chicken embryo fibroblast to isolate CAV virus. What is a suitable protocol to follow?

Protocol for using chicken embryo fibroblast for CAV virus isolation.

05 June 2015 3,842 2 View

Is there a published method for titration of chicken anemia virus in SPF chicks?

Titration of chicken anemia virus from artificially infected SPF chicks. Infection material is emulsion from field case. Compatible cell line aside from MDCC-MSB1.

30 April 2015 8,779 6 View