Filippo Massa

3 Questions 7 Answers 0 Followers

Questions related from Filippo Massa

Is 6xHis tag compatible with therapeutic (GMP) protein production?

Hello! We are producing a recombinant protein (from mammal cells) and we need a 6xHis tag for the purification. This protein is aim to be used in preclinical experiments in the short-medium term,...

12 January 2023 624 2 View

Is it possible to flip aminoacid sequences?

Goodmorning, I have a biomolecular dilemma that i can't answer. We would like to produce a fusion construct that is composed by two proteins in tail-to-tail conformation, otherwise one of the two...

06 January 2022 1,913 8 View

Is MicroPlex Library Preparation kit from Diagenode a reliable method for preparing proper libraries from few chromatin as starting material?

I'm planning to sequence low amount of Chipped chromatin: the only commercial kit adapted for very low amount of starting material is the Diagenode Microplex Library preparation kit. Does anyone...

21 July 2015 2,734 2 View