Fernanda Cerqueira

2 Questions 3 Answers 0 Followers

Questions related from Fernanda Cerqueira

Can I transfect and infect my cells at the same time?

I need to overexpress my protein and I normally transfect the cells with mitochondrial Ds-Red for microscopy. Can I do it at once? Of course I could try Mitrotacker Green Staining as an...

09 September 2016 3,505 2 View

Which DRP-1 antibody has being successfully used for immunoprecipitation?

How much of cell lysate should I use? I had a negative outcome using 600 ug of total protein and DRP-1 monoclonal antibody from BD (2 ug of antibody). I am using the IP kit from Pierce (which has...

02 February 2016 3,222 2 View