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Questions related from Fernanda Cortez Lopes
We are working with a recombinant peptide of 3 kDa, but we do not have tricine in our lab, only glycine. Is that possible to detect this peptide without using tricine in the SDS-PAGE? High...
30 November 2021 7,074 4 View
I am working with a MMLV-RT (Moloney murine leukemia virus- Transcriptase Reverse). I am expressing in E. coli pTGroE and purifying in a Chelating Sepharose loaded with Ni2+. I am eluting the...
02 June 2020 4,230 4 View
We are working with a protein that is expressed in inclusion bodies. We are using 4 M urea to solubilize, however when we start the refolding using dialysis and decreasing concentrations of urea,...
15 September 2018 9,209 5 View
I would like some help about the newest methodologies used to identify plants at species level. DNA barcoding seems to be the most used methodology.
02 January 2017 8,081 5 View
I would like to extract proteins from soybean callus, then I am looking for a protocol to obtain high amounts of protein. I would like to quantify a protein using ELISA methodology after the...
13 April 2015 5,800 3 View
I need to prepare a crude extract of Penicillium herguei to evaluate the activity of an intracellular enzyme. Do you think maceration is a good technique?
31 January 2014 4,486 3 View
My peptide is a plant peptide and it's expressed in E.coli BL21
13 June 2013 6,826 3 View