Fernanda Cortez Lopes

7 Questions 12 Answers 0 Followers

Questions related from Fernanda Cortez Lopes

Is that possible to detect a 3 kDa peptide using SDS-PAGE without tricine?

We are working with a recombinant peptide of 3 kDa, but we do not have tricine in our lab, only glycine. Is that possible to detect this peptide without using tricine in the SDS-PAGE? High...

30 November 2021 7,066 4 View

Someone has a suggestion to improve the purification of MMLV-RT protein?

I am working with a MMLV-RT (Moloney murine leukemia virus- Transcriptase Reverse). I am expressing in E. coli pTGroE and purifying in a Chelating Sepharose loaded with Ni2+. I am eluting the...

02 June 2020 4,224 4 View

Someone can suggest a efficient methodology to solubilize and refold a protein expressed in inclusion bodies?

We are working with a protein that is expressed in inclusion bodies. We are using 4 M urea to solubilize, however when we start the refolding using dialysis and decreasing concentrations of urea,...

15 September 2018 9,205 5 View

I am looking for new molecular methodologies to identify plants at species level. Someone could help me?

I would like some help about the newest methodologies used to identify plants at species level. DNA barcoding seems to be the most used methodology.

02 January 2017 8,077 5 View

Does anyone know an efficient protocol to extract proteins from soybean callus?

I would like to extract proteins from soybean callus, then I am looking for a protocol to obtain high amounts of protein. I would like to quantify a protein using ELISA methodology after the...

13 April 2015 5,795 3 View

Anyone knows a protocol to perform the extraction of intracellular enzymes from filamentous fungi?

I need to prepare a crude extract of Penicillium herguei to evaluate the activity of an intracellular enzyme. Do you think maceration is a good technique?

31 January 2014 4,481 3 View

Could you suggest a technique for quantifying protein expression?

My peptide is a plant peptide and it's expressed in E.coli BL21

13 June 2013 6,821 3 View