Fergus Morris

2 Questions 5 Answers 0 Followers

Questions related from Fergus Morris

Agarose EMSA for aptamer binding: no bound complex band?

Hey there, Due to limited resource, we are trying to assess the interaction of a 50mer aptamer with a 25kDa protein domain. The protocol we are using is unusual in that the gel is 2.5% agarose...

24 July 2020 8,291 8 View

Design of PCR primers for extremely short oligonucleotides?

Hello there, hoping you are well, I have reached a point in my current project where I need to amplify very very short (50mer) non-coding ssDNAs. I understand the options I have available...

21 July 2020 1,699 5 View