Fatemeh Razaghi

5 Questions 6 Answers 0 Followers

Questions related from Fatemeh Razaghi

How can I transfect neurons?

Hi all, I really need your help. I want to transfect glutaminergic neurons with ASO and siRNA, but I have no idea which reagent and method can help me more. I have wild type and mutant neurons,...

17 December 2024 8,116 4 View

Does anyone know why we use LB+CAM instead of LB+KAN after transformation steps in CRISPR-Cas9?

Hi. Does anyone know why we use LB+CAM instead of LB+KAN after transformation steps in CRISPR-Cas9?

04 December 2021 4,501 1 View

Would you please help me to find a way how to calculate serial dilution in one special microbial protocol?

Hi. 1.Pipette 0.9mlsterile saline (0.9% NaCl w/v) into each of six Eppendorftubes and label them.We recommend making a diagram in your notebook showing the procedure and the meaning of your...

03 November 2021 3,777 1 View

Does anyone knows how I can find more information about molecular mechanism of Fusarium oxysporum pathogenesis, treatment, virulence or drug resistanc

Hi. Does anyone knows how I can find more information about molecular mechanism of Fusarium oxysporum pathogenesis, treatment, virulence or drug resistance?

01 January 1970 5,852 3 View

High Resolution Melting Curve Analysis (HRM)

Hey, I would like to know more about HRM analysing. can anyone assist me regarding the analysing the data which were taken from this experiment?! I have used MeltDoctor reagent and I calibrated...

01 January 1970 9,054 5 View