Fareed Sairi

6 Questions 27 Answers 0 Followers

Questions related from Fareed Sairi

Share with me some tips to differentiate cells with cell debris with similar flow cytometry profile?

Currently, I'm trying to isolate a cells that produce a protein that localized near the cells membrane. This is mix cells from tissue. I had stained the cells with primary antibody+FITC conjugated...

09 September 2013 1,585 1 View

How to prevent clumps/aggregation of cells during permeabilization for FISH experiment?

I'm currently having a problem with clumps of cells after cell permeabilization treatment using 70% ethanol. I want to permeabilize suspension cells and treat with FISH probes. After that, the...

06 June 2013 8,488 8 View

Cell was not stained by Hoechst (NucBluestain from Invitrogen). Any reason?

I'm confused with my immunofluorescence result. I have mixed cells from mollusc mantle tissue. It was stained with protein pAb/FITC antibody. However, when I stained with Hoechst, those which gave...

10 October 2012 4,094 10 View

Does anyone know the fetal bovine serum electrophoresis profile? (Native PAGE)

I'm really interested to know more about proteins in FBS. Recently, I ran Native PAGE with FBS and I saw a number of bands at the range between 700KDa-800KDa. Does anyone know what kind of protein...

09 September 2012 4,557 3 View

Trypan blue destroying cells during counting. Why?

I performed cell counting using trypan blue on my cell culture. however, the cells were destroyed in less than 2-3 min after mixed. I had salts in the media. Has anybody got an idea why?

05 May 2012 7,798 21 View

Can anyone give me some tips or guide to develop abalone cell line? (specifically in media formulation and parameter condition)

There are low number of cell line/cell isolation/cell identification/cell culture in abalone. Most report were focused on hemocytes or mantle cells to produce pearl crystal. Even that type of...

03 March 2012 7,950 4 View