Farah Fatihah

5 Questions 12 Answers 0 Followers

Questions related from Farah Fatihah

How to dilute essential oil before antioxidant assay?

What are the solutes used to dilute essential oil for DPPH, TPC, FRAP and Beta/Carotene Bleaching Assay? Can I simply dilute the essential oil using 1 mg/mL in methanol/dH2O for all assay?...

22 November 2023 2,580 10 View

What is the possible reason why no emulsion formation in beta carotene/linoleic acid bleaching assay?

The procedure that I used 2 mg beta carotene in 10 mL chloroform with 200 mg Tween 40 and 20 mg linoleic acid. Next, chloroform removal by rotary evaporator and 50 mL of distilled water is added.

09 November 2023 1,863 2 View

What is the standard for Beta carotene/linoleic acid bleaching assay?

What is the standard/positive control that can be used instead of Butylated hydroxytoluene (BHT) or Butylated hudroanisole (BHA)? Sample: Plants’ essential oils

07 November 2023 2,224 8 View

How to plot standard calibration curve for FRAP assay?

Is it by using the inhibition (%) formula or simply plot a standard curve using absorbance and concentrations? If using the inhibition (%) formula, Is it correct if the negative control color is...

06 November 2023 5,200 2 View

How to prepare ascorbic acid standard for DPPH assay?

Can i use the usual calculation M1V1=M2V2 formula for this? And can I use 0.1 mg/ml for the ascorbic acid stock solution preparation?

27 October 2023 2,750 3 View