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Questions related from Ewan St. John Smith
Hello! We have been using the Select-HA MegaLadder and HiLadder from Hyalose/Amsbio, but Hyalose seem to no longer be producing them. On a quick flick through, nearly everyone seems to have...
09 September 2018 6,293 3 View
In the past I've used TEM to count myelinated and unmyelinated fibres in peripheral nerves (e.g. sciatic nerve), but in my current institution this is looking like it would be very expensive and...
07 July 2017 743 1 View
Hi all, I would like to set up a system for culturing active osteoclasts and I was wondering what people would recommend for a substrate? I know that dentin and bone can be used, but I was...
03 March 2016 8,803 3 View
Hello! I am planning on using siRNA knockdown in neurones to knock down expression of ion channel X. Alongside measurements of ion channel X function following control and targeted siRNA...
02 February 2015 4,998 5 View
We are using Lumafluor red retrobeads to label DRG neurones, which works brilliantly when recording from neurones in culture. However, for immunohistochemical studies we observe high background...
11 November 2014 480 9 View
Hi there, We are interested in getting in situ hybridisation up and running in our lab for looking at bits of brain and spinal cord. We are currently trying to decide if we should invest in the...
09 September 2014 8,635 11 View
I have used dextran Alexa 488 3,000 MW from Life Technologies/Molecular Probes for tracing nerve fibres innervating the paws of mice and found that 3 days post-injection into the foot pad is...
03 March 2013 5,391 33 View
