Erik Maronde

6 Questions 83 Answers 0 Followers

Questions related from Erik Maronde

Can anyone recommend beta1- and/or beta2-adrenergic receptor antibodies for IF/IHC?

I am looking for experience with beta-adrenergic receptor antibodies. Which worked and which not. Especially in brain tissue. I checked the elder literature but these antibodies are mostly no...

22 November 2022 1,282 5 View

Does anyone know what structure this is?

I found this structure on a kind of flintstone at the shore of a bathing pond in Northern Germany. Does anyone know what kind of structure that is? Skin? A leaf?

02 October 2016 6,524 12 View

Is mouse hippocampal neuronal differentiation in vitro comparable to in vivo?

Many groups use newborn mouse hippocampal cell preparations to analyse differentiation and signaling processes. These cultures are usually kept cultivated for 2 to 4 weeks in vitro before use. Do...

29 October 2014 1,744 5 View

Has anyone seen effects of forskolin on protein phosphorylation without measurably elevated intracellular cyclic AMP (cAMP) levels?

There is some evidence that forskolin does other things than activating adenylate/adenylyl cyclase in the literature. But has someone seen forskolin inducing protein kinase activity without...

05 August 2013 7,235 6 View

Are there specific or selective activators and/or inhibitors for cyclic nucleotide gated ion channels for use in a cell-culture system?

I observe a clear effect with forskolin and cyclic nucleotide-dependent protein kinase activators (Sp-cAMPS, SpcDDBIMPS), but cannot inhibit that with myr-PKI. The EPAC-specific agonist has no...

27 July 2013 2,549 24 View

Is myr-PKI a good PKA inhibitor in cell culture?

I treat cells with forskolin and measure CRE-luc reporter gene activity (which is elevated 30 to 50 fold by 10 µM forskolin). Rp-cAMPS and 8Br-Rp-cAMPS (both 1 mM) inhibit this nicely. Myr-PKI...

19 September 2012 918 12 View