Erik Harvey-Girard

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Questions related from Erik Harvey-Girard

Has anyone have tips on how to use and handle silk fibrion?

I want to replicate Jackman et al. (2018) technique. They mixed theirAAV virus suspension with silk fibrions, deposited it on the tip of an lens implant, and let it dry. Then, they performed the...

01 April 2019 3,488 0 View

How red blood cells protect themselves from ferroptosis?

They have load of iron which must induce lipid peroxydation via ferroptosis !!! What prevent them to burst quickly? The classic theme about the fact they don't have a nucleus is about physical...

17 August 2017 9,786 1 View

Should I use K gluconate or K methanesulfonate in patch solution?

I'm doing patch-clamp recordings. Somebody suggested that I use K methylsulfonate instead of K gluconate. Why would that be better?

15 May 2015 4,884 7 View

What is the best camera for IR-DIC to do patch-clamp?

I want to do patch-clamping on fish brain slice and I have difficulty to make seals. Osmolarities from both solutions are fine. Dissection is looking really good, my cells seem reasonable healthy....

08 September 2014 4,736 4 View

How can I improve the neuron survival rate in slice elecrophysiology?

The goal is to do patch electrophysiology in a telencephalon fish slice. However, the neurons (in DC and DD) die within an hour after dissection. The dissection is performed quickly in ice-cold...

30 July 2014 9,132 15 View