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Questions related from Erica Weber
Hello, I’m running a cell binding assay in a 96-well plate, and I need to incubate the cells for about 1-2 days. After leaving them overnight, they look stressed, I’m not sure why? I have the...
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I know plasma cells and plasmablasts have specific markers (CD138) and there are commercial kits available to isolate them. But is there a marker that can be used to isolate memory B cells? And...
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I'm having trouble eluting my 30 kDa fusion protein - despite trying lower expression temperatures, different colonies, and verifying the correct sequence and elution buffer. The protein remains...
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How can I measure the concentration of antibodies secreted by B-cells in culture? I need to quantify the antibodies in the supernatant without interference from other cell components. What’s the...
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How do I isolate plasma cells from blood? What’s the best method for pulling out B-cells (especially antibody-secreting cells) from blood samples?
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Hello, I'm curious to know - when we keep track of cell passages because they’re expressing a gene we care about, and if they’re passaged too many times, they lose that expression. Does the same...
01 January 1970 348 3 View
If I inject mice with wild-type cancer cells that naturally express multiple genes, we know their immune systems will produce antibodies against nearly all the genes present in those cancer cells....
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I understand that B cells mature in the bone marrow and are released as naive B cells, which express BCRs (B cell receptors). They remain inactive until they encounter their specific antigen, at...
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