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Hello, I’m running a cell binding assay in a 96-well plate, and I need to incubate the cells for about 1-2 days. After leaving them overnight, they look stressed, I’m not sure why? I have the...
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I know plasma cells and plasmablasts have specific markers (CD138) and there are commercial kits available to isolate them. But is there a marker that can be used to isolate memory B cells? And...
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How do I isolate plasma cells from blood? What’s the best method for pulling out B-cells (especially antibody-secreting cells) from blood samples?
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Hello, I'm curious to know - when we keep track of cell passages because they’re expressing a gene we care about, and if they’re passaged too many times, they lose that expression. Does the same...
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If I inject mice with wild-type cancer cells that naturally express multiple genes, we know their immune systems will produce antibodies against nearly all the genes present in those cancer cells....
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