Ellango Ramasamy

4 Questions 12 Answers 0 Followers

Questions related from Ellango Ramasamy

RNA Seq illumina PE reads - Reverse reads (R2) has poor quality?

While trimming the adaptor and low quality RNA-Seq illumina paired end reads in Trimmomatic, I have got more Forward only survive of about 40 to 50%. This study is for estimate the transcript...

29 November 2022 5,798 3 View

HTSeq2 - count Warning Message?

Hi Friends, while I run my RNA-Seq data (Tophat/HISAT2 aligned output & samtools name sorted file) in HTSeq2 for counting DEG, which display warning message like “Mate records missing for...

07 July 2017 9,454 3 View

Which package is better for calculate DE genes?

HI, Currently I am using cuffdiff for DE gene analysis in 2 condition (sample and treatment). Is there any package support for my work better!

06 July 2017 8,387 25 View

DEG analysis without biological Replication!!!

HI friends, Is it possible to analysis the differential expression genes/transcripts form RNA-seq without any replications!!!. Actually I am handling the year old plant transcriptome data of one...

29 June 2017 7,641 11 View