7 Questions 11 Answers 0 Followers
Questions related from Eleanor Martin
From what I've read these cells are already Hygromycin-B resistant. I was wondering if it is still possible to select for stably transfected cells using a really high concentration of hygromycin?
05 May 2019 6,843 1 View
New to crystallography...I want to compare ligand binding in a crystal structure to one generated using NMR. I have tried the pdbsum and the pisa servers but the hydrogen bonds that are identified...
12 December 2016 4,001 3 View
I am looking to create a stable cell line using Calu-3 cells. My initial plan is to co-transfect with pBABE-puro and select with puromycin. Does anyone have any similar experience with Calu-3? I...
08 August 2016 2,563 5 View
My POI is a human protein. However we when we had the DNA sequence synthesized we codon optimised for S.cerevisae (for protein expression). However, I now wish to do some interactome studies in a...
07 July 2016 5,946 4 View
Hello all, I want to fix a Superose 6 10/300 GL column to our AKTA prime system. However, the product info says that the Superose 6 10/300 GL is not suitable for use with the AKTA prime plus...
03 March 2016 5,489 5 View
I am working with a construct containing only the C-terminal region of my POI. It is 42 residues long, and contains no aromatic residues (but does contain a number of basic amino acids). Following...
11 November 2015 9,895 4 View
I recently attempted to cleave the tag of my protein of interest using Sigma's recombinant enterokinase E4906. However following negative purification, all the protein still eluted in the elution...
04 April 2015 8,032 3 View
