Eduard Fidler

9 Questions 15 Answers 0 Followers

Questions related from Eduard Fidler

Is PDI in DLS size analysis (peak width/peak height)^2 or (standard deviation/peak height)^2 ?

Hi All, I am just curious about this because I have seen a lot of people talk about pdi=(sigma/peak height)^2 but this attached paper talks about the other formula, which is used when? Thanks, EF

17 March 2016 3,748 6 View

Around what pH would antibodies tend to lose their activity?

Hi All, I am working with mAbs that are being exposed to a low pH (around 3 at the lowest) and was just wondering if that might be enough to cause them to lose some activity or even denature....

15 March 2016 6,347 3 View

Can anyone give some advice concerning mAb conjugation to Nanoparticles using Maleimide-Thiol chemistry?

Hi All, I am working on conjugating mAbs to the surface of PLGA-PEG-MAL nanoparticles. Free thiols are added to the mAbs using Traut's reagent, which will then be added to the NP suspension. I am...

13 March 2016 8,806 7 View

Would the acidity caused by TCEP*HCl reduction be able to denature/deactivate an antibody?

Hi All, I want to reduce coupled Anti-EGFR mAbs for use in active-targeted nano-constructs. I want to use TCEP*HCl as my reducer but know that it causes (and actually needs) an acidic pH when used...

13 March 2016 7,385 10 View

What are the effects of polymer size/MW on Nanoparticle formation?

Hi All, I am wondering how using polymers of differing molecular weights would affect the formation/properties of nanoparticles and why these differences would come about. Thank you! EF

01 February 2016 5,769 6 View

How many antibodies per Nanoparticle would result in effective targeting of an antigen?

Hi All, I am working on conjugating Anti-EGFR antibodies to the surface of PLGA-PEG-maleimide nanoparticles to target tumor cells over-expressing EGFR. My question is approximately what ratio of...

28 January 2016 3,069 4 View

What could happen to Diethyl Ether when it expires?

Hi All, I am using Diethyl Ether in a synthesis which has been failing. The ether is a few weeks past expiration and I was just wondering what that could mean chemically and whether that could be...

01 September 2015 7,009 3 View

Why might my EDC-NHS coupling of PLGA to PEG be failing?

Hi All, I have been struggling to successfully conjugate PLGA copolymer to PEG using EDC-NHS. I have followed a protocol using chloroform as a solvent, a 5:1 ratio of EDC/NHS to PLGA for the...

24 August 2015 5,433 5 View

Can I measure the concentration of antibodies conjugated to a nanoparticle without measuring those adsorbed to its surface?

Dear all, The antibody is Cetuximab and I am tagging it with Alexa Fluor 488. Some of the antibodies will adsorb to the nanoparticle surface (PLGA-PEG-MAL) instead of forming the thioether bond I...

05 March 2015 2,836 2 View