Dr Siddikuzzaman

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Questions related from Dr Siddikuzzaman

Is any one using 5-bromo-2'-deoxyuridine (BrdU)/ Click-iT® EdU Microplate incorporation assay for primary culture???

I am checking cytotoxicity of drug molecules by using ascites or tumour tissues. Challenge is reproducible data as primary have passage limitation and cells number too limited for so many...

14 February 2019 9,368 0 View

What is the alternative way to find out cytotoxicity of a drug/molecules/compound(s) for primary culture derived from Ascites and disease tissues??

We are using MTT, Colonogenic & SRB assay to check the cytotoxicity. MTT and colonogenic is not reproducible (may be, cell killing is totally depend on mode of action of a drug/molecules and...

13 February 2019 2,601 7 View