Dr Siddikuzzaman

4 Questions 12 Answers 0 Followers

Questions related from Dr Siddikuzzaman

Is any one using 5-bromo-2'-deoxyuridine (BrdU)/ Click-iT® EdU Microplate incorporation assay for primary culture???

I am checking cytotoxicity of drug molecules by using ascites or tumour tissues. Challenge is reproducible data as primary have passage limitation and cells number too limited for so many...

14 February 2019 9,451 0 View

What is the alternative way to find out cytotoxicity of a drug/molecules/compound(s) for primary culture derived from Ascites and disease tissues??

We are using MTT, Colonogenic & SRB assay to check the cytotoxicity. MTT and colonogenic is not reproducible (may be, cell killing is totally depend on mode of action of a drug/molecules and...

13 February 2019 2,647 7 View

Which is the effective and easy way to isolate epithelial cells from fibroblast/mixed cells in primary culture?

Can anyone please navigate me? Thanks

13 February 2019 8,403 4 View

In the process of inflammation, what are the factors that lead from localized inflammation to a systemic spill?

what factor decide that fate of localize inflammation to systemic or spill over ??

08 June 2015 1,724 5 View