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Questions related from Dong-Myung Kim
In trying to getting more effective neoantigens for cancer therapy, I have some questions as followings. 1. T-cell activation is considered to be more important than B-cell activation. Which...
02 February 2020 5,788 1 View
In our experiments, we found the single band of PCR product that matches the amplicon size of target gene-specific primer in acrylamide gel analysis. However, we still concern that non-target PCR...
11 November 2016 6,584 7 View
I am interesting to generate cell lines with GFP proteins which is inserted into a specific target gene and want to know if is is possible to generate the cell lines with...
07 July 2016 841 1 View
We are planning to perform qPCR in 384 or 1536-multiwell plates. However, the total reaction volume seems to be too small (2.5-100nl) to carry out qPCR. Practically, It seems to be very...
07 July 2016 1,915 8 View
I am now working with qPCR for quantifying the gene copies of each genes from a pooled culture of yeast. In designing the primers for each gene for qPCR. I'm searching for the answers of the...
12 December 2014 6,602 3 View
Is staining time important? If the answer is yes, I want to know if the damaged mitochondria by drugs are less-stained with the dye? Also, what are the detailed conditions such as dye density,...
11 November 2014 7,023 4 View