Dong-Myung Kim

6 Questions 5 Answers 0 Followers

Questions related from Dong-Myung Kim

A few questions about neoantigens for cancer therapy?

In trying to getting more effective neoantigens for cancer therapy, I have some questions as followings. 1. T-cell activation is considered to be more important than B-cell activation. Which...

02 February 2020 5,770 1 View

Would you please recommend the best method for analyzing the presence of non-target PCR products having same size in PCR reaction mixtures?

In our experiments, we found the single band of PCR product that matches the amplicon size of target gene-specific primer in acrylamide gel analysis. However, we still concern that non-target PCR...

11 November 2016 6,575 7 View

A question about exchagning a target gene with GFP in cell lines using CRISPR technology?

I am interesting to generate cell lines with GFP proteins which is inserted into a specific target gene and want to know if is is possible to generate the cell lines with...

07 July 2016 834 1 View

what is the best method for adding the primers and other mixtures for qPCR with many different samples simutaneously in multi-well plate?

We are planning to perform qPCR in 384 or 1536-multiwell plates. However, the total reaction volume seems to be too small (2.5-100nl) to carry out qPCR. Practically, It seems to be very...

07 July 2016 1,907 8 View

What is the method or kit for the genomic DNA preparation from yeast for qPCR?

I am now working with qPCR for quantifying the gene copies of each genes from a pooled culture of yeast. In designing the primers for each gene for qPCR. I'm searching for the answers of the...

12 December 2014 6,593 3 View

Is it possible to measure mitochondrial activity in drug treatments comparatively with mitotracker staining?

Is staining time important? If the answer is yes, I want to know if the damaged mitochondria by drugs are less-stained with the dye? Also, what are the detailed conditions such as dye density,...

11 November 2014 7,015 4 View