5 Questions 15 Answers 0 Followers
Questions related from Dmitry Klokov
Can someone recommend and method/way to calculate a proper number of cells for NGS? E.g. let's say we know that in the experiment we have 1 out of 2000 with a variant, and it is not present in the...
18 March 2019 8,999 2 View
Has anyone had luck with NGS library prep for DNA/RNA samples that had low A260/230 ratio due to guanidine thiocyanate? Our samples are made using the AllPrep Qiagen kit without Trizol (used other...
10 January 2019 6,373 4 View
I need an advice from a pathologist or a person who is experienced/familiar with histology of intestinal tumors in mice / APCmin mouse. See attached H&E slide please. We see sometimes these...
15 January 2018 1,732 4 View
Hi, I'm new to this field, but need to generate a list of miRNAs that target (experimentally validated) a specific list of genes from my experiments. Preferably in batch mode, i.e. list of genes...
03 September 2015 7,068 7 View
We are going to run an assay in which a Fluorescein-labelled plasmid is spotted on a hydrogel (3% polyacrylamide) coated slides and then imaged. I was wondering how sensitive the final...
28 April 2014 4,961 1 View
