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Questions related from Dmitry Klokov
Can someone recommend and method/way to calculate a proper number of cells for NGS? E.g. let's say we know that in the experiment we have 1 out of 2000 with a variant, and it is not present in the...
18 March 2019 8,986 2 View
Has anyone had luck with NGS library prep for DNA/RNA samples that had low A260/230 ratio due to guanidine thiocyanate? Our samples are made using the AllPrep Qiagen kit without Trizol (used other...
10 January 2019 6,360 4 View
Hi, I'm new to this field, but need to generate a list of miRNAs that target (experimentally validated) a specific list of genes from my experiments. Preferably in batch mode, i.e. list of genes...
03 September 2015 7,059 7 View
We are going to run an assay in which a Fluorescein-labelled plasmid is spotted on a hydrogel (3% polyacrylamide) coated slides and then imaged. I was wondering how sensitive the final...
28 April 2014 4,951 1 View