Divya Bhatia

2 Questions 8 Answers 0 Followers

Questions related from Divya Bhatia

What are the cells,those are RORgt+ IL-17-, gated from Th cells (CD3+CD8-)?

I have been looking at Th17 cells. I usually stimulate my PBMCs with PMA,ionomycin and monensin, perform surface as well as intracellular staining for Th17. Gate down CD3+CD8- cells (mainly Th,...

30 November 2013 4,253 6 View

What are RORgt+ IL-17- cells, gated from CD3+ CD8- helper T cells?

I have been looking at Th17 cells. I usually stimulate my PBMCs with PMA, ionomycin, and monensin, perform surface and intracellular staining. Here is my gating strategy: Gate down CD3+CD8- cells...

30 November 2013 4,808 7 View