17 Questions 39 Answers 0 Followers
Questions related from Diego Fernández-Fernández
Hi everyone, I am trying to record EPSCs on medium spiny neurons from corticostriatal afferents in the dorsal striatum. The problem is that most neurons I patch show very small EPSCs or no EPSCs...
05 May 2023 7,592 2 View
Hi everyone, Could someone explain why the combination of ketamine/xylazine is preferred (vs pentobarbital) to perform transcardiac perfussion for the preparation of striatum slices (for...
19 January 2023 9,868 4 View
Dear colleagues, I am planning to perform patch-clamp recordings using striatum slices from mice (young and adult). I have been checking the methods from different papers and my impression is...
17 August 2022 10,015 5 View
Hello everyone, I am performing some fEPSP recordings to study LTP on hippocampal slices. I am using a submerged chamber to maintain my hippocampal slices. However, my baseline is very unstable,...
29 April 2021 1,430 9 View
Hello everyone, I am trying to record single channel events on my HEK293 cells stably expressing Cav2.2 channels, using the cell-attached patch clamp technique. I used the following...
14 May 2020 4,181 1 View
Dear all, I am dealing with the common noise problem during my experiments. All of a sudden a very large, irregular and annoying noise arose on my setup, making my recordings literally...
27 November 2019 6,257 7 View
Hi everyone! I have trying to figure out how to determine if the number of functional Cav2.3 channels is different under my test conditions. These channels are stably expressed in HEK293 cells....
14 January 2019 5,574 0 View
Hello everyone, I am trying to record calcium currents from my HEK cells. Problem is that I don´t have much experience with series resistance compensation that is needed for such large...
20 September 2018 1,248 4 View
Hello everyone, We are trying to establish a primary cell culture from the parasympathetic ganglia of the mouse heart. However, although we get to see some neuron-like cells in our culture, we...
10 January 2018 7,099 0 View
Hello everybody. I have seen through publications that some labs have been routinaly able to induce very long-lasting in vitro LTD in young and adult animals. I would like to ask you if you can...
28 May 2015 548 13 View
I am having trouble to replicate one of the main postulates about the expression of late LTP, concerning its dependence on de novo protein synthesis. I performe fEPSP recordings on the Schaffer...
14 April 2015 3,164 5 View
I am planing some experiments to study difference in basal synaptic transmission accross different animal conditions. I am thinking about producing a fiber volley vs fEPSP slope curve, but I...
23 January 2015 3,038 5 View
Dear colleagues, In the near future I must perform a series of experiments with a compound which is highly sensitive to the mechanical movement induced by bubbling the ACSF. So we deliberatly need...
28 September 2014 6,879 4 View
I am looking for a reliable and powerful compound to validate a positive effect enhancing LTP in Schaffer collateral-CA1 experiments (rat hippocampal slices). I am performing both E-LTP (HFS 100...
13 June 2014 5,092 3 View
After having so many trouble with the signals I got in my rat hippocampal slices (small amplitude, poor inhibition, tendency for epileptic responses, often contamination with population spikes) I...
28 March 2014 3,831 25 View
I am trying to validate LTP experiments in 7 weeks C57BL mice. Hippocampal slices (400 µm). Measurements of the slope of the fEPSP (Schaffer collateral-CA1 synapse). I am not sure about how well...
13 February 2014 4,152 14 View
I am performing fEPSP recordings (Schaffer collateral-CA1) in slices from rat hippocampus, and I find so much difference in the PPF ratio from one experiment to another. Why is that? Sometimes I...
11 June 2013 848 38 View
