Dennis Pantazatos

4 Questions 16 Answers 0 Followers

Questions related from Dennis Pantazatos

What is a good cutoff value for a PLGS ladder score?

I'm running PLGS to identify peptides from an online pepsin digest using PLGS. I usually use Sequest and I am familiar with the scoring system (Xcor values etc.) PLGS gives these ladder scores and...

12 June 2014 2,024 0 View

Does protein fragmentation occur during MALDI Imaging and to what frequency

We are working on a project of identifying a 3232.56 Da unique peak identified from tissue imaging mass spec. Conventional LCMS analysis with an orbitrap and MASCOT searching does not produce a...

29 November 2012 1,238 10 View

Reducing a protein for LCMS analysis?

Does anyone have any suggestions for the use of an alternative method for reducing a protein then performing LCMS analysis? In our experiments we focus on a single protein to perform HDX studies...

18 July 2012 2,137 17 View

Does anyone have an opinion on whether the Thermo Q Exactive or a Waters SynaptG2-S is a better investment and the strengths & weaknesses?

Our core is deciding between the purchase or lease of a Thermo Q Exactive or Waters Synapt G2-S. I wanted to get feedback from the community on personal experience using the two instruments on...

04 May 2012 3,525 20 View