Deepak Mukundan K V

5 Questions 2 Answers 0 Followers

Questions related from Deepak Mukundan K V

Is there any recommended method for growing SH-SY5Y cells on Poly-D-lysine or Poly-L-lysine treated coverslips?

I am experiencing difficulties seeding SH-SY5Y cells on Poly-D-Lysine or Poly-L-Lysine treated coverslips.After 2-3 days, the cells either detach or fail to attain their typical morphology. The...

02 February 2024 3,719 1 View

Is there any specific Lysis buffer for protein extraction from neuronal cells?

Hii, I am currently using RIPA buffer for the extraction of proteins from neuronal cells. But, getting very faint bands on the blot. Initially, I was doubtful about reagents and the protocol...

09 December 2021 2,166 1 View

How to put Overlay histogram in Flowing software with different peak height?

Dear All, I have a doubt regarding flowing software.If anyone using the software kindly help me. My problem is while combining all the individual histograms, peak height of each individual one...

23 April 2021 9,288 3 View

Is it possible to ligate two restriction ends those have no complementary bases?

In my lab,we are trying to construct a control plasmid. So we have to remove the insert gene which has two restriction ends:one is XhoI and other one is Hind III.So after double digestion we want...

02 January 2021 3,493 4 View

Is there any options to make the applied biosystem's 96 or 384 real time plate compatible with light cycler II (roche)?

In our lab we are using roche's light cycler II for real time PCR. But the current issue is,we have ordered the plates for real time from applied biosystems. Lot of opinions are coming regarding...

28 March 2017 1,819 3 View