Danila Leontyev

3 Questions 2 Answers 0 Followers

Questions related from Danila Leontyev

Human T-cell mouse humanization protocol suggestion?

Dear colleagues, Can anyone share their experiences and insights regarding the optimization and improvement of human T-cell mouse humanization protocols? What modifications have you found to be...

30 January 2023 7,592 0 View

Does leukocyte gate shifts between samples in humanized mouse flow cytometry analysis?

Hello, We had a discussion on whether the leukocyte population can shift between different samples on the FSC / SSC plot and should the gate be adjusted on individual samples therefore? We are...

31 May 2022 4,492 1 View

What is the best gating strategy to count enriched CD34+ cells?

Hello, For our humanized mouse model we are isolating CD34+ cells from cord blood units using MACS kit. We found CD34+ purity about 98% after MACS enrichment. However, when frozen and thawed our...

22 October 2021 2,577 3 View