Dana Patino

4 Questions 1 Answers 0 Followers

Questions related from Dana Patino

FACS Fusion Sorting block waste reservoirs clogged?

lately our waste reservoirs in the sorting block keep getting clogged. I’m not sure what’s going on? we haven’t been able to use the instrument because the sheath fluid accumulation causes the...

08 September 2022 7,741 0 View

Heavy chain (mRNA) fluorescence labeling before single cell sorting cells?

Has anyone tried to label their mRNA to select the desired population before doing a sort? I was trying to figure out if there is a way I can assess roughly gene copy number differences in my...

11 October 2019 2,120 0 View

Sorting negative cells from GFP dim parental, please help?

I have an established cell line that expresses GFP constitutively. Once I transfect the cell line, there shouldn't any GFP expression since the DNA payload doesn't have a fluorescence marker. My...

24 September 2019 4,082 1 View

FACS-related Co-op project, can someone help me with their input?

Good evening - I need to come up with a project idea for co-op. The project needs to be mostly FACS-related but also give exposure to other techniques, such as cell culture and molecular biology...

19 September 2019 9,043 2 View