Dan Matthew Espinoza Burias

4 Questions 7 Answers 0 Followers

Questions related from Dan Matthew Espinoza Burias

How do I measure the zone of inhibition in antibiotic overlay assay?

What is the standard for measuring the zone of inhibitions in antibiotic overlay assay (i.e. isolates spot-inoculated on agar then overlaid with soft agar with the test strain)? There could be two...

21 November 2016 8,536 4 View

How do I perform extraction on mass cultivated anaerobe and microaerophiles in anoxic conditions?

Suppose that I can mass culture an anaerobic or microaerophilic bacteria from mangrove sponge, are there any specific protocols for extraction using ethyl acetate in anoxic conditions? Or will the...

11 January 2016 7,698 1 View

Does anyone know how to isolate pure metabolites from mangrove sponges?

Mangrove sponges live in association with a diverse microbial (mostly bacteria) communities. These bacterial communities also have their own secondary metabolites. I am asking for opinions on how...

29 April 2015 3,773 6 View

Does anyone know an/any enzyme(s)(bacterial) that hydrolyzes linear forms of beta-glucose monomer units into a polymer?

Is it possible to hydrolyze the -CHO head and -OH tail of two monomer units, forming a linear stand of ß-glucose for isolation further processing (serial organic reactions) for carbyne formation

18 April 2015 6,906 4 View