D. Temel

5 Questions 6 Answers 0 Followers

Questions related from D. Temel

How to make transfection of HEK293 for protein purification?

Hello, I want to transfect adherent HEK293 cells for protein purification. Which reagent/protocol is most suitable?

07 July 2019 3,096 4 View

How to get docking coordinates from MOE for RMSD calculation?

Hello, I want to calculate RMSD values for the docking in R programme, so I want to get the cordinates from the MOE and load to R. How can I get the data from MOE? Thanks in advance

06 June 2018 1,396 4 View

Which ß-Arrestin assay kit is better?

I want to test the functionality of the ß-Arrestin 1 and 2 after tagging with fluorescent proteins. Which assay kit do you use and recommend?

04 April 2018 9,070 0 View

Restriction enzyme digestion difference in agarose gel?

Hello everyone I want to insert a 60bp fragment into my vector and we used primers(that includes our insert and 2 RE cut site) after the ligation I want to make sure that my vector contains the...

03 March 2018 8,862 12 View

What are Golgi targeting sequences?

Hello everyone, I want to localize EGFP to the golgi by adding a targeting sequence. I cannot decide which sequence is really working when we cloned to the plasmid. Can anyone with an experience...

02 February 2018 7,288 3 View