Cristiane Naffah De Souza

4 Questions 9 Answers 0 Followers

Questions related from Cristiane Naffah De Souza

Is it possible to get mature neutrophils from immature neutrophils in vitro?

I would like to know if is possible to get mature neutrophils from bone marrow add G-CSF or other substance. Anybody have already done it?

12 December 2014 9,558 6 View

Why do I have problems extracting RNA from mouse neutrophils?

I have tried to isolate RNA from mouse neutrophils but my yield is very low, impossible to work with it. I started with 2 million neutrophils! Could someone help me? Thanks a lot

11 November 2014 9,557 9 View

What protocol can I use to isolate mature mouse neutrophils from bone marrow?

I performed a Percoll protocol (gradient 72%-62%), but I observed 2 rings, one on the surface and another between the gradients. Which is the ring that represents mature neutrophils?

05 May 2014 8,269 1 View

I need to do a infection in DO11.10 (murine cells). Is it possible to use phoenix ampho to do the transfection or do I need to use phoenix eco?

DO11.10 is a murine T lymphocyte hybridoma.

01 January 2013 1,266 3 View