Coralia Dehelean

3 Questions 2 Answers 0 Followers

Questions related from Coralia Dehelean

Can I replace mercaptoethanol with DTT in MSP Buffer?

Mercaptoethanol is toxic. Does anyone have experience with replacing it with DTT in buffer for methylation specific PCR? Thank you!

13 October 2021 2,416 1 View

Does DNA methylation in non-CpG regulate gene expression?

Hello, everyone! It is known that DNA methylation in promoter regions containing CpG islands is responsible for downregulation of genes. How about genes which don´t contain CpG islands in their...

29 April 2021 3,693 3 View

Can I predict miRNA by knowing the mRNA target?

Hello! I am trying to predict in silico the miRNA by knowing the target. Most of published research starts by knowing the miRNA and predicts the target using online tools. Are you familiar with...

01 January 2021 7,570 5 View