Cláudia Oliveira

3 Questions 5 Answers 0 Followers

Questions related from Cláudia Oliveira

Does anyone know if vesicle or lipidic samples could interfere with chromogenic assays for endotoxin detection?

I've tested an endotoxin detection kit in samples with a lipidic nature and have gotten a standard dilution measurement that was acceptable but OD readings that were quite inconsistent in these...

28 January 2022 2,371 0 View

Does anyone has suggestions to extract carbohydrates from E. coli?

Dear colleagues, We are interested in extracting carbohydrates fro E. coli and the protocol we've seen so far include too many hours in a sonicator. Does anyone has suggestions or experinece in...

27 November 2020 6,078 3 View

What condition should be prepared to induce proliferation in HUVECs to have it as positive control?

Dear colleagues, I've been trying to do proliferation assays using HUVECs and at the beginning we were using 20 ng/mL of VEGF as it was recommended by a colleague. However, this colleague uses...

11 October 2020 6,096 4 View