Christopher Mackerron

5 Questions 8 Answers 0 Followers

Questions related from Christopher Mackerron

Cre dependent vs non-Cre dependent DREADDS

Can somebody please explain to me the difference between these two? I'm aiming to inhibit neuronal firing with Clozapine N Oxide (CNO) after the successful transfection and expression of a G(i)...

01 January 2016 3,220 5 View

Are the problems with recording neuronal action potentials using Di-3-ANEPPDHQ, filter or sampling rate issues?

Try to make this brief, currently trying to record action potentials across a neural network, using Di-3-ANEPPDHQ, a voltage sensitive dye which blue shifts during excitation of the cell. My...

04 April 2015 6,672 9 View

What are the effects of UV on the PDMS backbone?

Hey there folks, will try to be brief. I grow neurons on micro-fluidic devices made from PDMS, however due to experimental conditions, I can not use anti-biotics. In order to create a sterile...

01 January 2015 9,069 9 View

Suitable PGL4.10 reverse primers - can anyone help?

Hey there, I've looked on Promega for suitable PGL4.10 reverse primers, however, it's only providing me with RVprimer3 and RVprimer4. Assuming these are forward primers (as it does not specify),...

07 July 2014 2,892 4 View

Are harshly split H9C2 cells still viable for transfection?

Hey guys, just some background information first:Growing H9C2 cells and trying to ensure they maintain the phenotye of Cardiac cells. They are grown in T150 flasks, maintained and grown in 25ml of...

07 July 2014 8,718 3 View