Christina-Nefeli Kontandreopoulou

4 Questions 6 Answers 0 Followers

Questions related from Christina-Nefeli Kontandreopoulou

ELISA kit for measuring serum anti-glycoprotein E antibody concentrations in samples after Shingrix vaccination?

Hello to everyone, I am currently conducting a study on immunogenicity of Shingrix vaccine in hematological patients. After a research in recent publication I noticed that an in house ELISA is...

29 March 2024 4,259 0 View

How do I identify CpG islands in order to design primers?

Hello everyone, I want to proceed to quantification of methylation of RRM1 gene with restiction enzymes and Real-time PCR. I need to design primers for the CpG sequence. RRM1 gene...

05 November 2021 6,572 2 View

Should I dilute human serum samples for ELISA?

I recently performed a competitive ELISA for nAbs to SARS-CoV-2. Samples values were over ranged, so they need dilution. Since the diluent from the company is not enough for that (Wantai...

07 October 2021 8,139 26 View

How do I manage a pellet of dead cells during thawing procedure for FACS acquisition?

It is my first attempt with Flow cytometry (acquisition) and I am having a problem during the thawing procedure. I work with PBMCs and I have to manage with a pellet of cells which I cannot...

06 October 2021 4,373 4 View