Christian Baumgartner

7 Questions 9 Answers 0 Followers

Questions related from Christian Baumgartner

How do you perform QRT-PCR from hematopoietic stem cell fractions?

I actually plan to do RNA isolation/cDNA/QRT-PCR from sorted hematopoietic stem cell fractions. Does anybody have experience in this? How can I downscale the number of cells used for RNA...

03 March 2014 5,956 8 View

How to do FACS on primary mouse hepatocytes?

We want to do some FACS analysis on freshly isolated primary mouse hepatocytes. So far, we had problems with high autofluorescence. Any suggestions to solve this problem? Can the isolation method...

01 January 2014 293 9 View

Can anyone help with the background differences in C57/B6 mice for bone marrow transplantation experiments?

We are planning to perform bone marrow / hematopoietic stem cell transplantations in C57/B6 mice and I have a question: there are different C57/B6 strains - C57Bl/6-J and C57Bl/6-N. Besides...

11 November 2013 5,629 9 View

Sorting of mouse hematopoietic stem cells?

I sorted bm LSK cells and got around 15,000 cells from 4 isolated bones (femur + tibia). We are interested in LSK CD150+ CD48- subfractions as well as CMPs, GMPs, MEPs, afterwards we want to...

09 September 2013 6,815 8 View

Quantification of mouse hematopoietic stem cells and progenitors per femur bone?

I am trying now for a couple of months to quantify the absolute numbers of LSK cells, LSK CD150+Cd48- and also CMPs, GMPs and MEPs. Especially for the stem cell enriched fractions like LSK cells I...

09 September 2013 4,276 8 View

Phosflow technique to study signaling in hematopoietic stem cells?

I want to study intracellular signaling in mouse hematopoietic stem/progenitor cells. Due to the fact that stem/progenitor cells are a very small population, it would be cool to use phosflow...

09 September 2013 5,387 4 View

Using anti mouse CD16/32-PE for FACS analysis - which blocking reagent?

I would like to characterize my hematopoietic progenitors using CD16/32-PE vs CD34 APC. CD16/32 is the FcyRII/III to characterize CMPs, GMPs and MEPs in mouse bone marrow. Usually, I use...

09 September 2013 1,368 1 View