3 Questions 10 Answers 0 Followers
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Most protocols seem to just state 3 hours of growth.
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Currently we use novex 4-12% bis-tris gels from life technologies. We can run them at 200V in MES buffer, which takes 35 minutes to run. I was wondering if there was a protocol to hand make gels...
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My cells don't seem to be growing and getting infected. The first batch I tried look like they were frozen with infection. I then got a supply from another lab and it has been a week and they...
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