Chinnamani Prasanna Kumar

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Questions related from Chinnamani Prasanna Kumar

What all are the factors responsible for higher and lower read numbers of 16S rRNA and COI gene, respectively of same species in an eDNA sample?

The barracouta (Thyrsites sp.) and the krill Nyctiphanes sp., exhibited the highest read number in every sample for the targeted 16S metabarcoding assay in Jeunen et al., (2018) study. However,...

08 August 2019 1,312 5 View

Is there any specific primer for amplifying cbbM genes of ammonia oxidizing bacteria (Beta-, and Gamma- classes of proteobacteria) ?

Can anyone suggest any articles that have published cbbM primers for ammonia oxidizing bacteria? Elsaide and Naganuma et al (2001) primers could amplify all autotrophs, but I need to focus on...

12 December 2014 3,278 1 View

How much quantity (ng/microlitre) of DNA (after PCR) would be optimum for clone library construction?

need to make sure the process work, I want to be sure that the amplicon I obtained was good enough for clone library construction. Hence I wish to know much much quantity of PCR product...

08 August 2014 4,294 4 View