Chiao-Jung Han

4 Questions 3 Answers 0 Followers

Questions related from Chiao-Jung Han

Some SRR accessions cannot be downloaded by fasterq-dump in the SRA-toolkit?

Hi all, I want to download some reference microbiome data from NCBI sequence Read Archive (SRA). I previously used fasterq-dump or fastq-dump to download the data, and the data were used to be...

08 August 2022 7,815 1 View

Is it possible to conduct a microbiome analysis using both HiSeq and MiSeq V3V4 data together?

Hi all, I want to compare my microbiome data with previous research. My data is sequenced by MiSeq platform (300 bp paired end), whereas the previous research only have Hiseq2500 data (250 bp...

25 June 2022 1,678 3 View

Denaturing Gradient Gel Electrophoresis (DGGE)- Why do my samples barely move into the gel?

I was trying to use DGGE to separate different microbes. I used 8% Acrylamide/Bis-acrylamide gel with 10%-40% denaturant and ran 280 mins (130 V), and the DNA seemed to move into the gel a little...

02 July 2021 2,418 2 View

Does the gut microbiome tend to become less diverse when the insect host (or any invertebrate) harbors one or more obligate intracellular symbionts?

Hi guys, I've been reading the book of "insect symbiosis" and some related journal articles. One article says that: "they kept pea aphids under aseptic conditions (the aphid is completely free...

01 January 1970 2,660 0 View