Chen Wen-Jie

5 Questions 3 Answers 0 Followers

Questions related from Chen Wen-Jie

How to permeabilize zebrafish larva in TUNEL assay experiment?

I want to detect apoptosis on zebrafish larva (3~5 dpf), so I conducted a TUNEL assay experiment following the instructions provided by the supplier on-line (In Situ Cell Death Detection Kit,...

11 January 2018 7,504 2 View

Why is that long Aβ peptides (Αβ1-42) always migrate faster than the short ones (Aβ1-40) in a SDS-PAGE or urea gel ?

As far as I know, SDS-PAGE separates peptides by the molecular weight variation and the longer a peptide is, the slower it migrates. However, I've read a lot of paper which demonstrated long...

21 September 2017 2,848 1 View

Can RNAlater be used to preserve tissue samples for at least a year ?

I have to preserve brain tissues of zebrafish for at least one year before I use these samples to do microarry . I heard that RNAlater can do this ,but I'm not so sure. Is there anybody has used...

06 November 2016 2,523 1 View

Are there antibodies specifically binding zebrafish endogenous tau protein , and its phosphorylated forms ?

I want to check if a KO mutation of nicastrin gene in zebrafish increase tau hyperphosphorylation by western blot. But I can't find antibodies that specifically target zebrafish endogenous tau...

14 October 2016 6,817 3 View

Is exclusion of zebrafish with low locomotivity necessary in a T-maze behavioral test ?

I'm going to conduct behavioral test with T-maze on zebrafish at different ages.When I used a few samples to go through the behavioral test protocol, I found there were some fish have difficulty...

07 October 2016 3,858 3 View