Chen Tian

3 Questions 1 Answers 0 Followers

Questions related from Chen Tian

Is there any bioinformatic tool can predict gram-negative bacteria based on 16S gene sequences (except BugBase)?

I am currently trying to identify gram-negative bacteria from my 16S metagenomic sequencing data, whose OTU/ASV is picked by Usearch. Somehow I found the OTU table can't be operated by BugBase...

15 September 2023 610 1 View

How to test the accuracy of popular source contribution calculation like FEAST/Sourcetracker 2?

I've been using these two calculations to find out the potential source of the microbiome in the air, but none can tell their calculation accuracy and I would like to know the reliability of the...

26 February 2023 8,704 0 View

Why in most research papers, the DNA standard for fungi qPCR is preffered to be cloned themselves other than purchased from commercial product?

I'm doing qPCR for fungi ITS2 region, which uses ITS3KYO2 and ITS4 primer pair. But I get stuck on getting the sequence of that region for purchasing commercial DNA standards. Most of my...

31 May 2022 5,097 0 View