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CRISPR/Cas9-mediated HDR-induced Knock-in (KI) in zebrafish seems to yield very low efficiency in germ-line transmission. To obtain a higher efficiency, we may need to optimize several factors...
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I fused mCherry into the C-terminus of transferrin receptor (TfR) which is a type-2 transmembrane protein (2292bp). So, mCherry supposed to express out side of the cell. During the confocal...
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Although there are several lines of evidences suggesting the expression of MHC class II in activated mouse T cells the acceptance seems to be controversial. Please help me for any confirmation...
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