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Questions related from Cédric Chaveroux
Hi all, I'm optimizing immunoprecipitation against a flagged protein. For this, I have cells overexpressing my tagged protein and a control with just the empty vector. I have tried four different...
08 October 2019 2,354 6 View
Hi, I saw that growing cells on ultra low adherence plates is similar to soft agar assays. However, if i'm not wrong, making spheroids on these plates requires stem-like cells whereas soft agar...
14 November 2018 5,437 0 View
Hi everyone, We are trying to optimize a protocole for cell cycle analysis in 6-well plates. We prefer this format of plates because it is what we use regularly for siRNA transfections. So we...
23 November 2017 2,516 7 View
Hi all, I'm trying to clone a shRNA in the pLKO-tet-on (21915, Addgene). So far, few clones have been obtained, but one issue is the quantity of plasmid i get from miniprep for screening. It is...
09 March 2017 1,144 11 View
Hi all, I have a cell model expressing luciferase that I would like to use for xenograft. The luciferase activity will be used for monitoring tumors growth by measurements with an IVIS...
22 September 2015 7,903 4 View
Hi everyone, I'm planning to do shRNA cloning in the pLKO.1 for lentivirus production. i read the protocol provided by addgene et some others. There is a little that I don't understand regarding...
30 March 2015 9,503 4 View
Hi all, Just a naive question. I compared the IF of couple of journals and the SJR. I still don't get the real difference between the two. Furthermore I noticed that, in SJR, they provide a...
01 January 1970 274 0 View
