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Questions related from Cédric Chaveroux
Hi all, I'm optimizing immunoprecipitation against a flagged protein. For this, I have cells overexpressing my tagged protein and a control with just the empty vector. I have tried four different...
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Hi, I saw that growing cells on ultra low adherence plates is similar to soft agar assays. However, if i'm not wrong, making spheroids on these plates requires stem-like cells whereas soft agar...
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Hi everyone, We are trying to optimize a protocole for cell cycle analysis in 6-well plates. We prefer this format of plates because it is what we use regularly for siRNA transfections. So we...
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Hi all, I'm trying to clone a shRNA in the pLKO-tet-on (21915, Addgene). So far, few clones have been obtained, but one issue is the quantity of plasmid i get from miniprep for screening. It is...
09 March 2017 1,106 11 View
Hi everyone, I'm planning to do shRNA cloning in the pLKO.1 for lentivirus production. i read the protocol provided by addgene et some others. There is a little that I don't understand regarding...
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