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Questions related from Catherine Anne Kemme
Hi all, working on some HIFI assemblies and wondering if anyone could give me some tips. I am struggling with getting an insert of about 2.5 kbp to ligate to my vector of interest. I have done...
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Hi All, This may sound like a stupid question, but I have a yeast transformation protocol that I am trying to modify for automation. Unfortunately, our automated equipment is not under any sort...
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I am currently using the Wizard genomic DNA extraction kit from Promega to extract DNA from Pichia yeast. However, when I measured the extracted DNA from the kit, I got around 400 ng/ul as...
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We have a certain protocol that we follow in lab for a PCR test that we do with our DNA. We have been doing this same protocol for the past 2 years. For the first year, it has worked just fine....
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Can anyone explain why a gradient gel (bought from BioRad) would turn white when put in Coomassie staining solution? I have never had this problem before with gels. I have always added the...
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I am currently trying to extract a pCC1 vector from Epi300 cells with little success. I thought I successfully extracted the plasmid from the cells (although a low yield), but I am beginning to...
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